earticle

영어

The methanol extract of Arachis hypogaea shell showed antioxidative and antimicrobial activity. The methanol extract was successively purified by solvent fractionation, silica gel adsorption column chromatography, Sephadex LH-20 column chromatography and octadecylsilane column chromatography. The purified active substances were isolated by high performance liquid chromatography, and were identified as 3-methoxy-4-hydroxybenzoic acid and 4-hydroxybenzoic acid by LC-MS and GC-MS. The amount of 3-methoxy-4-hydroxybenzoic acid and 4-hydroxybenzoic acid were 3.8 mg and 9.8 mg per kg of shell, respectively.

한국어

땅콩(Arachis hypogaea)껍질의 MeOH 추출물은 항균 및 항산화 활성을 보여 땅콩껍질에 함유된 활성물질의 탐색을 시도하였다. MeOH 추출물을 solvent fractionation, silica gel adsorption column chromatography, Sephadex LH-20 column chromatography, ODS column chromatography 등으로 정제하고 HPLC에 의해 활성물질을 분리하였다. 분리된 두 활성물질은 LC-MS, GC-MS분석에 의해 4-hydroxybenzoic acid와 3-methoxy-4-hydroxybenzoic acid로 동정되었으며 두 물질은 땅콩껍질에 각각 3.96 mg/kg, 9.73 mg/kg정도 함유되어 있었다.