earticle

영어

A hepatitis B Surface Antigen(HBsAg)-screening kit using immunochromatographic assay(ICA) method was developed by employing two kinds of antibodies. One is mouse monoclonal anti-HBs for tracer antibody and the other is goat polyclonal anti-HBs for capture antibody. This capture antibody was immobilized on the surface of nitrocellulose(NC) membrane and the
tracer antibody was conjugated with gold particles. When serum sample was added to the sample well, the conjugates deposited in a dry state on the surface of glass fiber filter were reconstituted and then combined with HBsAg in serum. In 5 min after adding, the assay result was visible through the window, that is, the complexes composed of HBsAg and the
conjugates appeared as maroon line on the lower part of the NC membrane. The detection limit of the ICA kit was 2 ng/ml when being tested with the reference HBsAg.

한국어

면역크로마토그래피 진단방법을 이용하는 B형간염 스크리닝 kit를 개발하기 위하여 두가지의 항체를 이용하였다. 표식자항체로 사용된 것은 단세포군항체 anti-HBs이고 포획항체는 goat anti-HBs인데 포획항체는 니트로셀룰로즈 막에 고정되고 표식자항체는 금 입자에 결합된다. 혈청 검체를 well에
가하면 유리섬유 표면에 건조상태로 침착되어 있던 conjugate가 활성화되어 검체중의 HBsAg와 결합한다. 검체를 가한 지 5분 후 검사결과가 나타나는데 HBsAg와 conjugate가 결합된 복합체가 니트로셀룰로즈 막의 하단부에 붉은 색 선으로 나타난다. 본 kit의 검출한계는 표준 HBsAg 용액을 사용하여
시험하였을 때 2 ng/ml이었다.