earticle

영어

We propose a mediator-free glucose biosensor which use immobilized glucose oxidase on tin oxide. Direct electron transfer is possible, because the tin oxide shows redox properties similar to those of the mediators. The preparation method for tin oxide and glucose oxidase-modified tin oxide is also simpler than the existing methods. Tin oxide provides an efficient surface for the
immobilization of large amounts of enzymes, due primarily to the porosity of the surface. Glucose oxidase was immobilized on tin oxide via the entrapment method, using nafion. The suggested method provides a simple process for enzyme electrode fabrication. Glucose oxidase immobilized on tin oxide prepared in accordance with this method has a relatively large current response, as compared to those of other glucose biosensors. The sensitivity of the biosensor
was 39A/mM, and a linear response was observed between 0mM to 3mM glucose. The Michaelis-Menten constant was determined to be lower than in electrodes prepared via the methods in current use. Also, this biosensor shows good reproducibility and stability.