원문정보
초록
영어
Quantitative analysis of DNA microarray is one of important issues in mRNA expression profiling and estimation for infectious doses of pathogens1). Here, we newly introduced an artificial standard probe strategy for quantitative data analysis of pathogen detection oligonucleotide chip as a model system improved from our previous report2). The artificial standard probe was designed to have below 85% similarity with any bacteria in order not to occur non-specific hybridizations with other targets. Based on fluorescence intensities of artificial standard spots, we successfully demonstrated that raw fluorescence intensities of specific probe spots could be corrected to have quantitative correlations with target concentrations. Therefore, our suggested rtificial standard probe strategy may be used for correction of chip-to-chip variation and making
possible quantitative analysis of one-color oligonucleotide microarray experiment.