원문정보
초록
영어
We develop a micro analysis and detection system for replicase of the hepatitis C virus. The fabricated analysis system consists of a bead based microchip and a computer based controller. A microheater, a micro temperature sensor and a microreator for bead packing is integrated in one microchip to realize several sequences for the capture and the elution of replicase. In this study, we used the PEG-grafted PS bead on which RNA aptamers are immobilized and performed thermal denaturation for releasing target molecules, and analyzed by MALDI-TOF MS. We performed several experiments to optimize conditions. As a result, we could acquire several matched peaks (26 % sequence coverage) of HCV replicase using the following condition: at room temperature for 20 min of binding time and at 85℃ for 30s of thermal denaturation time.