earticle

영어

The binding interaction of Salmonella typhimurium was studied with bacteriophage (P22) ligand and antibody ligand. A locally constructed fluidics system was used to deliver solutions to the compact, three-channel SPRTEETATM sensor. The binding interactions between Salmonella typhimurium and bacteriophage (P22) in addition to antibody were measured using surface plasmon spectrum by monitoring a variation of the Response Unit (RU) values. The association and dissociation rate constants of Salmonella typhimurium with bacteriophage (P22) and antibody were determined from a kinetic model applied curve-fitting method. The affinities between Salmonella typhimurium with bacteriophage (P22) and antibody were also considered with their kinetic parameters. The affinity appeared in the binding of Salmonella typhimurium with bacteriophage (P22) ligand.1) Salmonella typhimurium also exhibited significant adhesive properties antibody ligand.