원문정보
초록
영어
White Spot Syndrome Virus (WSSV) occurs worldwide and causes up to 100% mortality within 7-10 days after the infection in commercial shrimp farm, resulting in large economic losses to the industry 1). Besides the economic impact of the disease, the natural aquatic ecology is also
threatened as a wide host range of WSSV among crustacean 2). WSSV contains at least five major structural protein and the VP28 is a major component of its envelope protein. Of the viral structural protein, the envelope proteins usually play very important roles in virus infection and
assembly. So the aim of this study is creating VP28 polyclonal antiserum and determining its WSSV neutralization ability for development of vaccines against WSSV. The VP28 ORF was successfully cloned in pCold I vector and expressed in E.coli BL21 . The purified VP28 protein was injected 3 times into a rabbit to produce VP28 polyclonal antibody. The polyclonal
antibody was confirmed by ELISA and the antiserum was prepared for the neutralization. To study the role of VP28 in WSSV infection in Penaeus chinensis shrimp, an in vivo neutralization test was performed. The result of this experiment showed that WSSV infection was neutralizated by the VP28 polyclonal antiserum and that VP28 is involved in the infection process of
WSSV. In further work, VP28 recombinant protein will be used for the production of vaccine against WSSV.