원문정보
초록
영어
The immobilization of lipases, using ionic liquids (ILs) and multi-walled carbon nanotubes (MWNT) as additives to protect the inactivation of lipase during sol-gel process, was investigated. Among the lipase co-immobilized with ILs, the lipase showed the highest hydrolytic activity when the most hydrophilic IL, [C2mim][BF4], was used as an additive, while the highest stability of lipase was obtained by using the most hydrophobic IL, [C16mim][Tf2N]. Therefore, the binary mixtures of these ILs as additives were used to increase both activity
and stability of immobilized lipase. The hydrolysis and esterification activities of lipase co-immobilized with the mixture of 1 : 1 were 10-fold and 14-fold greater than in silica gel without ILs, respectively. On the other hand, MWNT was used as an additive in the sol-gel process to immobilize lipase. The hydrolysis and esterification activities of lipase co-immobilized with MWNT were 6-fold and 5-fold greater than in silica gel without MWNT, respectively.
After 7 times reuse of the lipase co-immobilized with 0.33% MWNT, the residual activity of lipase was 93%, while the immobilized lipase without MWNT showed no activity.