원문정보
초록
영어
Quantum Dots-oligonucleotide conjugates, which are complementary to small region of target DNA, can probe interest microorganism in water environment. Luminescent Quantum Dots (QD) has narrower emission spectra and a continuous broad-band excitation compared with conventional fluorescent dyes (such as FITC and Cy3) so it has been used for many years for biological staining and diagnostics.1) It is easy to make QD-oligonucleotide conjugates by
incubating QD coated with streptavidin and oligonucleotides modified with biotin. After incubation reaction free oligonucleotides, the unbound with QD, have to be removed from the mixture because hybridization efficiency decreases if unbound (unconjugated) oligonucleotides coexist with QD-oligonucleotide conjugates in the hybridization reaction. This study represents the application of field flow fractionation (FFF) for enabling easy separation of QD-oligonucleotide
conjugates, which are required for effective-hybridizing to complementary target sequences. The FFF is one of the most versatile families of separation technique known. It has been successfully applied to studies of the environmental colloids, bacteria, proteins, and etc in a wide size range.2)3) The oligonucleotide used here is specific for Microcystis sp. to be able to produce microcystin and purified QD-oligonucleotide conjugates was applied to detect Microcystis sp. in the environmental sample.