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영어
Besides a role as a causal agent of leaf spot disease of timothy (Phleum pratense), the hypomycetous fungus Cladosporium phlei (C. T. Gregory) de Vries is now considered as a potential provider of a key intermediate, phleichrome, which is a fungal pigment belonging to a group of perylenequinone and known to be further converted to a photodynamic therapeutic (PDT) agent.1) In this study, protoplasts of C. phlei were prepared and transformed to hygromycin B resistance using a plasmid, which contains a 2.4 kb SalI fragment of pDH25. The transformation efficiency ranged from approximately 20-100 transformants per experiment. Southern blot analysis of stable transformants revealed integrative transformation of the vector at different sites and more than two copies of the inserted marker gene appeared to be common. The establishment of easy protoplast-mediated transformation method of C. phlei should greatly facilitate the various molecular breeding of this strain for better production of bioactive materials such as fungal pigment.