원문정보
초록
영어
Bacterial community shift was examined in relation with changes in process performance throughout a lab-scale anaerobic reactor treating whey permeate. The operation was successfully performed with the complete mineralization of initial substrate after 42 days of incubation. Bacterial and archaeal population dynamics were quantitatively monitored using real-time quantitative PCR (qPCR). Bacterial community structure was investigated using denaturing
gradient gel electrophoresis (DGGE) based on 16S rRNA gene. An Aeromonas-like microorganism was supposed to be responsible for the rapid fermentation of carbohydrate. Several Clostridium-related bacteria with putative roles in carbohydrate fermentation, lactate oxidation, ethanol oxidation, or homoacetogenesis were found. The statistical analysis of the DGGE profiles provided a comprehensive insight into changes in bacterial community structure.
The bacterial community in the reactor shifted anticlockwise and returned close to the initial location on the non-metric multidimensional scaling (NMDS) plot. In conclusion, these results suggest the possibility of process diagnosis and performance prediction by monitoring bacterial community1).