원문정보
초록
영어
To investigate the effect of low culture temperature on cell growth and recombinant protein production, Chinese hamster ovary (CHO) cells producing erythropoietin (EPO) were cultivated between 25ºC to 37ºC with perfusion mode. CHO cells were cultivated at 37ºC until viable cell concentration reached 1 x 107 cells/mL. After that, temperature was shifted to 25ºC, 28ºC, 30ºC, 32ºC, 37ºC (control), respectively. Among five perfusion cultures, perfusion at 32ºC seems to be optimal since it showed the highest specific EPO productivity (qEPO) and cumulative EPO production. In addition, the heterogeneity, antennary structure and charge profile of EPO were investigated after the secreted EPO during perfusion culture at various culture temperatures was purified with ion exchange chromatography. A detailed result will be discussed.