원문정보
초록
영어
Recent advances in toxicity biosensing technologies have drawn heavy attentions from not only professionals but also public. One of the obstacles for this technique to be in full blossom is the relative weakness in securing biological contents having specific functions such as stress responses. In this presentation, as a type of biological contents, stress-specific bioluminescent bacteria are presented and discussed in terms of how they are developed and utilized as biological contents. The impacts of toxic chemicals on Escherichia coli could be analyzed using oligo DNA chips. The promoters of the specifically up-regulated genes from differential gene expression data of DNA microaray were inserted into a vector containing promoterless luxCDABE and transformed with E. coli strains, and numerous biosensing bacteria, therefore, have been finally constructed successfully. More than forty different recombinant bioluminescent bacteria having different promoters mentioned above were used for toxicity detection and classification through their specific bioluminescent response after fabricated in a form of cell array biochips (1, 2) or a multi-channel continuous mode. The fast toxicity detection and analysis is possible by using these biosensors/ biochips. These biosensors and biomonitoring systems could be implemented for high-throughput analysis of newly synthesized chemicals and drugs in addition to environmental toxicity analysis.