원문정보
초록
영어
Although there are many methods known for detection of the protozoan parasite Cryptosporidium parvum in water, such as immunofluorescence or PCR techniques, they are labor-intensive and time-consuming sensor systems. To overcome these problems, label-free biosensors for this pathogen is highly desirable. Surface plasmon resonance (SPR) biosensor systems offer one alternative as label-free systems. A major advantage of SPR detection is that it does not require any labeling or staining of the analytes, and that the binding can be monitored in real-time.1,2) In this study, the SPR biosensor based on mixed self-assembled monolayers (SAMs) was developed for real-time detection of Cryptosporidium oocyst. To enhance the sensitivity of real-time detection, the modified flow type SPR biosensor was developed, which consisted of the
immunoreaction step between the biotin-antibody and oocysts followed by the binding step of antibody bound oocysts onto the sensor surface. And also, a new real-time inhibition assay method was developed for detection of low concentration of C. parvum oocyst on the basis of flow type SPR biosensor.