원문정보
초록
영어
As the amount of genetic information exponentially increases due to recent advances in genome sequencing, the demand for a faster and more parallel expression system has become greater than ever. For this reason, cell-free protein synthesis has received renewed attention as an alternative method for high-throughput gene expression. In contrast to in vivo gene expression methods, where protein synthesis is carried out in the context of cell physiology and is surrounded by cell walls and membranes, cell-free protein synthesis provides a completely open system that allows direct access to the reaction conditions. At the same time, most of the cell functions other than protein synthesis need not be maintained in the cell-free system. Here we present our recent achievements in developing a highly efficient cell-free protein expression system. Through a series of systematic approaches to address the key issues affecting the efficiency of cell-free protein synthesis reactions, currently we can produce milligram quantities of proteins in a simple batch reaction. Based on this effective and robust platform for rapid production of recombinant proteins, we currently are seeking to combine the techniques of cell-free protein synthesis with numerous other disciplines.