earticle

영어

Enzymes are being widely used since it has many advantages, such as mild reaction conditions, substrate specificity. More than 3000 enzymes are known to work in cells and microorganisms, which means that enzymes can be used for 3000 different reactions in vitro. Especially, enzymes will play an important role in developing industrial biotechnology. Even though enzymes have enormous potentials for commercial applications in pharmaceuticals, food, bioelectronics, and chemicals industries, enzymes have also disadvantages to be solved; enzymes are in general expensive, the researches on enzyme applications have not yet been performed extensively, enzymes are not stable enough. Overproduction of enzymes using genetic engineering tools have been performed to reduce the enzyme cost. Screening of novel enzymes and improvement of enzymes using directed evolution and protein engineering have been performed to get better enzymes. Novel enzymatic reactions for bioenergy and chemicals production are currently very important issues nowadays. Immobilization of enzymes and novel enzyme reaction system are also regarded as important issues. In this presentation, the issues to be solved in enzyme engineering are presented and discussed along with recent efforts from academia and industries.