원문정보
초록
영어
To display β-galactosidase whose bacterial surface display is not easy due to its tetramer
formation and large size (116 kDa), Bacillus subtilis spore display system was developed. Among Bacillus subtilis spore coat proteins, eleven coat proteins were examined, and CotE and CotG were selected. SDS-PAGE analysis of proteins extracted from purified spore of Bacillus subtilis, FACS analysis of purified spore, protease accessibility test proved successful surface display of β-galactosidase on Bacillus subtilis spore. β-galactosidase displayed on Bacillus spores was used as a whole-cell immobilized biocatalyst for transgalactosylation in water-solvent biphasic reaction systems. Successful display of GFPUV and streptavidin using Bacillus subtilis spore can be used for various biotechnological application.