원문정보
초록
영어
The L-valine producing strain of Escherichiacoli was constructed by rational metabolic engineering and stepwise improvement based on transcriptome analysis and insilico gene knock-out simulation. The engineered L-valin producing base strain harboring pKKilvBN produced 1.31 g/liter L-valine. Comparative transcriptome profiling combined with in silico geneknock-out simulation was used for the enhanced production of L-valine. The VAMF strain (Val ΔaceF Δmdh ΔpfkA) harboring pKBRilvBNCED and pTrc184ygaZHlrp was able to produce 7.55 g/liter L-valine from 20 g/liter glucose, resulting in a high yield of 0.378 g L-valine per g glucose. The approaches described here can be a good example of developing strains for the enhanced production of amino acids based on systems biotechnology. [This work was supported by the Korean Systems Biology Project of the Ministry of Science and Technology (M10309020000-03B5002-00000). Further supports by the LG Chem Chair Professorship and KOSEF through the CUPS are appreciated].