원문정보
초록
영어
Diabetic nephropathy (DN) is a renal disease which develops as a consequence of diabetes mellitus (DM). Microalbuminuria is the earliest clinical sign of DN. There are no specific diagnostic biomarkers for DN other than microalbuminuria. However, microalbuminuria does not constitute a sole independent indicator for DN. Therefore, 2-DE was utilized to identify biomarkers for the more specific and accurate prediction of progressive nephropathy in type Ⅱ DM, via comparisons of the serum proteome in three experimental groups: type Ⅱ
diabetes patients without microalbuminuria (DM, n = 30), with microalbuminuria (MA, n = 29) and with chronic renal failure (CRF, n = 31). Differentially expressed proteins in MA and CRF groups as compared to those in DM group were identified by ESI-Q-TOF MS/MS and confirmed by Western blotting. Notably, RBP4 and eGPx were measured by ELISA using DM and MA patient
samples. The sensitivities and specificities (based on ROC curves) of RBP4 and eGPx were 84% and 83% respectively, and 88% and 73% respectively. Collectively, the results show that the three proteins identified in this study may constitute potential biomarkers for the diagnosis of type Ⅱ DN.