원문정보
초록
영어
We report a novel method to fabricate poly(vinyl alcohol) (PVA) hydrogel nanopatterns for constructing the nanoarray of lipid rafts as a form of tethered bilayer lipid membranes (tBLMs). Nanopatterns of UV-curable PVA were fabricated on gold substrates by ultraviolet nanoimprint lithography (UV-NIL). The surface of PVA hydrogel strongly restricted the adhesion of vesicles,
resulting in selective arrays into the nanowells (NWs) of PVA hydrogel. A vesicle fusion and a mixed self-assembled monolayer (SAM) assisted vesicle fusion methods were used to confine the tBLM-formed lipid rafts into the nanopatterned PVA hydrogel. The tBLMs were not formed into the PVA hydrogel NWs by a simple vesicle fusion method. Nanoarray of tBLM-formed
lipid rafts into PVA NWs has been accomplished by raft vesicle fusion onto a mixed SAM comprised of a mixture of 2-mercaptoethanol and a PEG lipopolymer. We expect that nanoarrayed lipid raft as a form of tBLMs provides an alternative platform for the analysis of transmembrane proteins, membrane-associated proteins, cell adhesion and a useful tool enabling the high-throughput applications such as nanodevices or nanochips.