원문정보
초록
영어
In modern science and technology, size matters, because materials exhibit new characteristics in nano scale, which do not exist in bulky state. Scientists have made great efforts to utilize the distinctive properties of nano sized materials especially for overcoming drawbacks of conventional sensors. Quantum dots are one of representing labeling nanoparticles which have photostability, size tunable color, broad exciting energy level and narrow emission spectrum. Other noticeable labeling agents are gold nanoparticles because of their high light absorbing efficiency. Therefore, I investigated the use of the strong optical signaling nanoparticles as
labeling agents for novel nanoscale materials such as DNA, proteins and carbon nanotubes. In particular, the nanoparticles were applied to develop simple homogenous assay modes. For these purposes, FRET was implied to both quantum dots and gold nanoparticles. FRET is controllable by engineering the interaction between targets and probe molecules. Molecular beacon and isothermal cycling probe technology were applied to engineering FRET on the nanoparticles for target specific signaling. Accordingly, I will presents the target detecting functions of the labeling nanoparticles and discuss functional effecting factors.