원문정보
초록
영어
We have been developing technologies for the rapid and automated identification and enumeration of microbial species that reside in environment critical to human health, such as water in the International Space Station. DNA Hybridization assays are widely used for biomarker detection for both research as well as environmental applications. We employ highly parallel DNA microarrays in direct profiling of microbial populations in complex samples, including monitoring bacteria. This project aims to create an integrated microfluidics system including automated cell lysis, fragmentation and labeling, and hybridization to a DNA probe microarray. A limiting factor of most hybridization assays, long hybridization time, can also be overcome by sample recirculation. Furthermore, fragmentation and labeling can be performed controllably in a single microfluidic device, which provides better consistency. Our technology could have far-reaching applications in clinical settings such as testing oral health.