원문정보
초록
영어
Cryopreservation of boar semen has increased the efficiency of the artificial insemination(A.I). The common cryoprotectant is glycerol which prevent to form internal ice crystals1). Although sperm remain a lot with it, it has a problem that reduces the fertility of sperm2),3),4). Therefore, it is needed to evaluate other cryoprotectants that might be less toxic than and as effective as glycerol. The cryoprotectants evaluated in this study were chosen according to their lone-pair
electrons and low molecular weight compared with glycerol5). It was studied that urea can act as a cryoprotectant as effective as glycerol when wood frog hibernate in winter6). Lactamide provided greater post-thaw sperm motility than glycerol, acetamide, or formamide7). Acetamide and lactamide produced pregnancy rates of 88 and 84% for frozen/thawed rabbit sperm8). Several recent studies had evaluated methyl formamide (MF) and dimethyl formamide (DMF) as alternative cryoprotectants for freezing equine sperm9),10),11). The aim of present study was
evaluating urea and several amides as cryoprotectants for freezing boar semen by comparing viability and DNA integrity.