원문정보
초록
영어
Neonatal diarrhea of pigs is caused by porcine Rota virus. Thus, the development of an optimum process for a safe and effective Rota virus vaccine is keenly required. For an effective vaccine, high virus titer is needed, and vaccine should be produced in a serum free medium for a safe vaccine. Therefore, the production of Rota virus vaccine was tried using MA104 cells in
a serum free medium, and the kinetics of cell growth and optimum condition for Rota virus production were investigated. Maximum virus titer of 6.39x106 pfu/㎖ was achieved by the addition of 0.2% trypsin 20 / ㎕ ㎖ at infection time and 3㎕/ ㎖ during post-infection period, with MOI of 0.1, infection time of 3 day, harvest time of 3 day. Cell growth kinetics showed that the maximum cell density with serum containing medium(RPMI1640 with 3% FBS) was about 25% higher than that with serum free medium(VP-SFM). Thus two approach; Rota virus production in no-serum medium (MEM-a) and serum free medium(VP-SFM) were tried. The maximum virus titer of 6.75x106 pfu/㎖ was observed in no-serum medium, and titer of 6.39x106 pfu/㎖ in serum free media. The optimum production strategy will be discussed based on virus titer and
operability of bioreactor, and optimum production conditions will be suggested.