원문정보
초록
영어
Rifamycin B, the product of the fermentation, is used as a raw material for the synthesis of rifampicin, chemotherapeutic agents used for combating tuberculosis, leprosy and AIDS-related mycobacterial infections1. The most efforts to improve the rifamycin B production were strain improvement by random mutagenesis. No systematic studies to choose suitable carbon and nitrogen sources and to optimize their concentrations were reported. Recently, the biosynthesis of rifamycin has been studied extensively to develop new drug based on rifamycin molecular structure by combinatorial biosynthesis2. However, the yield of metabolite intermediate is too low to be identified. Accordingly, development of optimal medium will be helpful to increase the yield of these intermediates. Here we present the study for screening of fifteen medium components including carbon and nitrogen sources by performing twenty experiments separately by a
Plackett-Burman design3. Glucose, malt extract, (NH4)2SO4, corn steep liquor, and KH2PO4 had confidence levels above 70%. Effects of malt extract, corn steep liquor, and KH2PO4 at two levels of concentrations were further examined at 30g/L of glucose and 5 g/L of (NH4)2SO4 in the following experiment. The positive effect of corn steep liquor was found whereas malt extract and KH2PO4 showed negative effect.