원문정보
초록
영어
Chiral vicinal diols are valuable synthetic intermediates for preparation of biologically active molecules because of their versatile reactivity. The epoxide hydrolase (CcEH) gene of Caulobacter crescentus was cloned by PCR and expressed in Escherichia coli. (S)-Styrene oxide was hydrolyzed into (R)-pheny-1,2 ethanediol via attack on the benzylic carbon of the epoxide ring by CcEH. CcEH attacks the less substituted carbon of (R)-Styrene oxide to
form (R)-phenyl-1,2-ethandiol (R)-Phenyl-1,2-ethanediol was obtained with more than 90% enantiomeric excess using recombinant CcEH at the optimal condition. Acknowledgement: This work was financailly supported by the Region Innovation System Program, Ministry of Commerce, Industry and Energy, Republic of Korea.