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논문검색

Production of Functional Hybrid Mussel Adhesive Protein FP-151 in Recombinant Baculovirus/Insect Sf-9 Cells

초록

영어

Mussel adhesive proteins have been suggested as a basis for environmentally friendly adhesives in aqueous conditions and in medicine. Previously, we produced functional recombinant hybrid mussel adhesive protein fp-151 that is a fusion protein comprising six-repeated fp-1 decapeptide repeats at both each terminus of fp-5 terminus. Here, we produced a recombinant fp-151, which was secreted in culture broth, in baculovirus/insect Sf-9 cell expression system, and compared its production and characteristics with E. coli-based fp-151. These assays showed recombinant fp-151 from insect cell culture has significant adhesive ability and may be useful as a bioadhesive in medical or underwater environments.

저자정보

  • Kyoung Ro Kim Department of Chemical Engineering, Pohang University of Science and Technology
  • Sunghye Lim Department of Chemical Engineering, Pohang University of Science and Technology
  • Youngsoo Gim Department of Chemical Engineering, Pohang University of Science and Technology
  • Dong Soo Hwang Department of Chemical Engineering, Pohang University of Science and Technology
  • Hyung Joon Cha Department of Chemical Engineering, Pohang University of Science and Technology

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