원문정보
초록
영어
Carboxypeptidase H (CPH) is a putative post-translational processiong enzyme, which removes basic amino acid residues from the intermediates during protein-hormone biosynthesis. We have isolated CPH cDNA clones from a flounder (Paralichthysolivaceus) brain cDNA library. The nucleotide and deduced amino acid sequence of the flounder CPH cDNA consisted of 1,590 base pairs encoded polypeptide of 455 amino acid residues, with a calculated molecular
mass of 50 kDa. Comparison of the structure and nucleotid esequence of the CPH genes from flounder, zebrafish and mouse have revealed that the CPH gene is highly conserved. The presence of the CPH mRNA species in brain was confirmed using the Northern blot analysis. A recombinant protein of flounder CPH gene was functionally expressed in E.coli. This result will provide a wider base of knowledge on the primary structure of the flounder CPH at the
molecular level and the functional diversity.