원문정보
초록
영어
This study was conducted to introduce a carboxybutyryl functional group to Glc and to find out the inhibitory mechanism of novel Glc derivative, carboxybutyrylated Glc (CGlc) on iNOS and COX-2 expressions in LPS-induced mouse macrophages (RAW264.7 cells). Production of NO and PGE2 was inhibitedby CGlc pre-treatment, and suggested a possibility of down-regulating
their respective genes, iNOS and COX-2. RT-PCR and western blot analysis revealed, CGlc can affect on both transcriptional and translational levels of above gene expressions. The data from NF- B promoter gene transfection κ experiment supported the idea that inhibition of iNOS and COX-2 is due to down-regulation of their transcription factor, NF-κB. Following stimulation with LPS, p38 MAPK and JNK present in upstream of NF-κB signaling were also inhibited by CGlc
treatment. However, the protein level of other MAPK, ERK remained unaffected. Therefore, it can be concluded that, CGlc is capable of inhibiting iNOS and COX-2 expressions in LPS-induced RAW264.7 cells via attenuation of NF-κB signaling by p38 MAPK and JNK but not by ERK.