원문정보
초록
영어
Avermectin is a secondary metabolite with powerful anthelmintic and insecticidal activities, thus it is used as an efficient agent in the field of agriculture and animal health. Avermetin can be produced by polyketide pathway in S. avermitilis. Also oligomycin is a polyketide compound produced by S. avermitilis. In this research, oligomycin production was disrupted to increase avermectin production. To create plasmid for disruption, the smallest gene of oligomycin
synthetase gene cluster was obtained by PCR from S. avermitilis chromosome.
Then, apramycin resistance gene was inserted in oligomycin synthetase gene for selection. After transformation of this plasmid, oligomycin synthetase gene (olmA5) in the chromosome was replaced with disruption cassette on the plasmid via homologous recombination. As a result of this gene replacement, we obtained mutants (olmA5::apra) that no longer makes the oligomycin compounds. And the mutants confirmed by PCR & HPLC analysis. Although no significant increment of avermectin production in the mutant strain was observed
This mutant can become a good source of strain improvement for better avermectin production because use of the mutant can simplify separation of avermectin, it can be resulted in saving time and cost as well as protect worker from hazardous sucstance(oligomycin) in industrial place