원문정보
초록
영어
The tylosin polyketide synthase (PKS) tylGI-V genes were cloned into two compatible replicating vectors each under the control of pikAI promoter. These two replicating plasmids, pBB155 containing tylGI-III and pDHS3003 containing tylGVI-V were individually introduced into an engineered strain of Streptomyces venezuelae bearing a deletion of pikromycin PKS gene cluster (DHS2001). The resulting mutant strain (YJ005) successfully produced the 16-membered ring macrolactone (tylactone) and its glycosylated form (desosaminyl tylactone). To improve the production level of tylactone and its derivative, endogeneous pathway specific regulatory gene, pikD was introduced and the resulting mutant produced 3.4-fold enhanced tylactone and 18-fold improved desosaminyl-tylactone. The result demonstrates the regulatory protein PikD triggered the enhanced expression of Tyl PKS as well as desosamine (des) biosynthetic gene cluster
to promote the productivity of tylactone and its glycosylated form.