원문정보
초록
영어
S-Adenosylmetionine(SAM) plays an important as methyl donor in proteins, DNA, RNA, phospolipid, polysaccharide and numerous molecules. Also, SAM play an pivotal function in the primary and secondary metabolism of cells1). However, little is known about mechanism of SAM synthetase in P.ciferrii. In order to examine the effect of SAM in P.ciferrii, we isolated SAM synthethase gene from it by low-strigency PCR, DNA Walking and RACE . The open reading frame (ORF) of SAM synthetase isolated from P. ciferrii was 1152bp and had a low G+C content of 32.12%. The highest similarity was to hypothetical protein SAM synthetase 2 gene of its close relative Debaryomyces hansenii , with 88% similarity. This isolated gene known as SAM synthetase gene includes consensus ATP-binding motif Gly-X-Gly-X-X-Gly and metal binding site (23D and 279D for Mg2+ and 51G for K+). In the recent research, SAM’s effect of morphological differentiation has been reported in several species2). First of all, we will confirm morphological differentiation in yeast, and we are studying secondary metabolism of P.ciferrii. Characterization of SAM synthetase from P.ciferrii is now in progress.