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High and Compact Formation of Baculoviral Polyhedrin-Induced Inclusion Body by Co-Expression of Baculoviral FP25 in Escherichia coli

초록

영어

Previously, we found that baculoviral polyhedrin (Polh) can successfully be used in Escherichia coli as a fusion partner for the expression of special foreign proteins as inclusion bodies, and the resulting, easily isolatable Polh-induced fusion inclusion bodies had almost the same characteristics as the native Polh. Here, we investigated the effects of co-expression of baculoviral FP25 protein on Polh-induced inclusion-body production in an E. coli expression system, as FP25 is known to be involved specifically in polyhedra formation. Using several analytical tools, including SDS-PAGE, pronase proteolysis, solubilization under alkaline conditions, and electron microscopy, we found that co-expressed FP25 was associated with Polh-induced inclusion bodies and that its co-expression led to formation of compact inclusion bodies as well as high production levels. We confirmed that FP25 co-expression induced higher production levels of other heterologous protein, antimicrobial peptide Hal18, fused with aggregation-prone Polh. Therefore, co-expression of baculoviral FP25 can be promisingly used to increase the levels of baculoviral Polh-fused foreign proteins, especially harmful proteins, expressed as inclusion bodies in an E. coli expression system.

저자정보

  • Lin Li Division of Molecular and Life Sciences, Pohang University of Science and Technology, State Key Laboratory of Agricultural Microbiology, School of Life Science and Technology, Huazhong Agricultural University
  • Young Soo Kim Division of Molecular and Life Sciences, Pohang University of Science and Technology, Department of Chemical Engineering, Pohang University of Science and Technology
  • Dong Soo Hwang Division of Molecular and Life Sciences, Pohang University of Science and Technology, Department of Chemical Engineering, Pohang University of Science and Technology
  • Hee Jeong Jung Division of Molecular and Life Sciences, Pohang University of Science and Technology, Department of Chemical Engineering, Pohang University of Science and Technology
  • Jeong Hyun Seo Division of Molecular and Life Sciences, Pohang University of Science and Technology, Department of Chemical Engineering, Pohang University of Science and Technology
  • Juan Du State Key Laboratory of Agricultural Microbiology, School of Life Science and Technology, Huazhong Agricultural University
  • Hyung Joon Cha Division of Molecular and Life Sciences, Pohang University of Science and Technology, Department of Chemical Engineering, Pohang University of Science and Technology

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