원문정보
초록
영어
To understand the cellular physiology under anaerobic glycolysis condition by overexpressing anaplerotic enzymes, which medicating carboxylation of C3 metabolites into C4 metabolites, in Eschericha coli, anaplerotic NADP-dependent malic enzyme (MaeB), as well as the other anaplerotic enzymes were artificially expressed and their C4 metabolism was compared in E. coli. Increasing MaeB expression enhanced the production of C4 metabolites by 2.4 times compared to the wild-type strain in anaerobic glucose medium with bicarbonate supplementation. Supplementing the medium with 10 g/L of NaHCO3 increased C4 metabolism by 3 times in cells with increased MaeB expression, which showed the greatest response to increased CO2 availability among the anaplerotic enzymes tested. The higher C4 metabolism was achieved in E. coli expressing increased levels of the NADPH-dependent MaeB. The greatest increase in the C4 metabolite ratio compared to the other tested enzymes were also found in E. coli with enhanced MaeB expression as CO2 availability increased. The physiological deviation depending on the anaplerotic overexpressions are discussed.