원문정보
초록
영어
Mannheimia succiniciproducens MBEL55E is a non-motile, non-spore-forming, mesophilic, and capnophilic gram-negative bacteria isolated from Korean Bovine. M. succiniciproducens can produce a large amount of succinic acid as a major fermentation product while producing succinic, acetic, and lactic acids as the major end-products. Based on the genome sequence, genetic engineering is required to reduce the production of other organic acids. However, cloning genes directly into M. succinciproducens is not reported so far. Therefore we constructed a shuttle vector pME19 which replicates stably in both Escherichia coli and M. succiniciproducens using the replicon of pMVSCS1. This shuttle vector can be used for further genetic engineering of M .succiniciproducens for succinic acid production.