원문정보
초록
영어
Mannheimia succiniciproducens MBEL55E isolated from bovine rumen is a capnophilic
rumen bacterium and an efficient succinic acid producer. Recently, a genetically engineered succinic acid overproducing mutant, Mannheimia succiniciproducens LPK7, was developed based on full genome sequence. Here we report the proteome reference map of M. succiniciproducens MBEL55E by analyzing whole cellular proteome, membrane proteome and secreted proteome. Based on these proteome reference maps, cells in the different growth phases of the wild type strain, MBEL55E, and its mutant, LPK7, were analyzed and compared. These comparative proteome analysis revealed valuable information to understand physiological changes during growth and succinic acid overproduction, and subsequently suggested target genes to be manipulated for the further strain improvement. [This work was supported by the
Korea Science and Engineering Foundation (KOSEF) grant funded by the Koreagovernment (MOST) (2005-01294). Further supports by the LG Chem Chair Professorship, IBM SUR program, Microsoft, and by the KOSEF through the Center for Ultramicrochemical Process Systems are appreciated.].