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논문검색

Comparative Response of Callus and Seedling of Jatropha curcas L. to Salinity Stress

초록

영어

Jatropha curcas L. is an oil bearing species with many uses and considerable economic potential as a biofuel crop. Salt stress effect on growth, ion accumulation, contents of protein, proline and antioxidant enzymes activity was determined in callus and seedling to understand the salt tolerance of the species. Exposure of callus and seedling to salt stress reduced growth in a concentration dependent manner. Under salt stress Na content increased significantly in both callus and seedling whereas, differential accumulation in the contents of K, Ca, and Mg was observed in callus and seedling. Soluble protein content differed significantly in callus as compared to seedling, however proline accumulation remained more or less constant with treatments. The proline concentration was ~2 to 3 times more in callus than in seedling. Salt stress induced qualitative and quantitative differences in superoxide dismutase (SOD; E.C. 1.15.1.1) and peroxidase (POX; E.C. 1.11.1.7) in callus and seedling. Salt induced changes of the recorded parameters were discussed in relation to salinity tolerance.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
  Callus culture and growth determination
  Germination percentage and seedling growth determination
  Determination of ion content
  Proline extraction and estimation
  Enzyme extractions and assays
 RESULTS
  Effect of salt on germination percentage and growthof callus and seedling
  Effect of salt on ion accumulation
  Effect of salt on protein and proline accumulation
  Effect of salt on the activity of antioxidant enzymes
 DISCUSSION
 ACKNOWLEDGEMENT
 REFERENCES

저자정보

  • Nitish Kumar Discipline of wasteland research, Central Salt & Marine Chemicals Research Institute (CSIR, New Delhi), Bhavnagar, Gujarat-364002, India
  • Meenakshi Kaur Discipline of wasteland research, Central Salt & Marine Chemicals Research Institute (CSIR, New Delhi), Bhavnagar, Gujarat-364002, India
  • D.V.N. Sudheer Pamidimarri Discipline of wasteland research, Central Salt & Marine Chemicals Research Institute (CSIR, New Delhi), Bhavnagar, Gujarat-364002, India
  • Girish Boricha Discipline of wasteland research, Central Salt & Marine Chemicals Research Institute (CSIR, New Delhi), Bhavnagar, Gujarat-364002, India
  • Muppala P. Reddy Discipline of wasteland research, Central Salt & Marine Chemicals Research Institute (CSIR, New Delhi), Bhavnagar, Gujarat-364002, India

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