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논문검색

Isoflavone-O-β-D-Glucan extract from the submerged-liquid culture of Agaricus blazei Murill block Cell Cycle Progression and induces apoptosis in human breast MCF-7 cancer cells

초록

영어

The isoflavone-O-β-D-Glucan extract is a potent chemical component in the extract from the submerged-liquid culture of Agaricus blazei Murill. In the present study, the anti-tumor effect of isoflavone-O-β-D-Glucan extract was investigated using MCF-7 cells. MCF-7 cells were maintained with Dulbecco's Modified Eagle Medium/Ham's F-12 nutrient (DMEM/F12) supplemented with 10% fetal bovine serum (FBS) at 37℃ in a humidified 5% CO2. For cell
proliferation experiments, cells were seeded in 90 ㎜ dishes, treated with the various concentrations (0, 1, 5, 10, 15, 20, 25 ㎕) of the isoflavone-O-β-D-Glucan extract (10 ㎎/㎖) for over a period of 96 hr of incubation. The isoflavone-O-β-D-glucan inhibited the proliferation of cells in a dose-and time-dependent manner, which was mediated through up-regulation of
p21/p53 and down-regulation of cyclin D. The results suggest that isoflavone-O-β-D-Glucan extract inhibited the proliferation of MCF-7 cells by induces apoptosis and block cell cycle progression in the G/S phase. This study was partly supported by the BK21 program of GNU.

저자정보

  • Yun Ah Kim Divesion of Applied Life Sciences, Graduate School, Gyeongsang National University
  • Young Suk Kim Divesion of Applied Life Sciences, Graduate School, Gyeongsang National University
  • Seon Min Lee Divesion of Applied Life Sciences, Graduate School, Gyeongsang National University
  • Wook Jin Jang Divesion of Applied Life Sciences, Graduate School, Gyeongsang National University
  • M. A. Islam Divesion of Applied Life Sciences, Graduate School, Gyeongsang National University
  • Su Jeong Shim Divesion of Applied Life Sciences, Graduate School, Gyeongsang National University
  • Jeong Ok Kim HK Biotech Co. Ltd.
  • Cherl Woo Park HK Biotech Co. Ltd.
  • So Young Kim Jinju International University
  • Eun Ju Lee Korea Food and Drug Administration,
  • Yeong Lae Ha. Divesion of Applied Life Sciences, Graduate School, Gyeongsang National University

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