원문정보
초록
영어
Reactive oxygen species (ROS) are constantly generated in aerobic organisms during normal metabolism in response to both internal and external stimuli. Imbalances in the production and removal of ROS have been hypothesized to play a causative role in numerous disease such as cancer, ischemia/reperfusion injury, and degenerative diseases such as photoaging, atherosclerosis, arthritis, and neurodegeneration. A feature often associated with these diseases is a malfunctioning of the connective tissue remodeling process due to increased activity of extracellular matrix metallo proteinases (MMPs). Algae extracts have been studied as potential natural antioxidants in recent years. The brown algae Sargassun thunbergii is distributed widely coastal area of east asia. In the present study, the free radical scavenging potency and
inhibitory effects methanolic extract Sargassun thunbergii (ST) on ROS production and MMP-2 and 9 activities were investigated. It's scavenging activity of ROS such as 1,1-diphenyl-2-picrylhydrazyl(DPPH), superoxide and alkyl radical was determined using electron spin resonance(ESR) spectroscopy. The levels of intracellular ROS was measured using
2,7-dichlorofluorescindiacetate. Their levels significantly decreased in the presence ST extracts, and the DPPH radical scavenging effects were higher than superoxide and alkyl radicals. Furthermore, MMP-2 and -9 expression levels were significantly decreased ina dose dependent manner by treatment of ST extracts. Therefore, these results suggest that ST extract could have a
therapeuticpotential for prevention and treatment of several diseases such as tumor metastasis, inflammation and neurodegeneration.