earticle

영어

Sea horse, Hippocampus coronatus (SH) a marine teleost fish, is well known not only for its special medicinal composition and used as one of the most famous and expensive materials of traditional Chinese medicine. It was extracted with water (SHW), methanol (SHM) and ethanol (SHE), respectively and evaluated by various antioxidant assays. The including total antioxidant, DPPH radical scavenging, hydroxyl radical scavenging, superoxide anion radical scavenging, alkyl radical scavenging, reducing power and protective effect on DNA damage caused by hydroxyl radicals generated. Further,the ROS level was detected using a fluorescence probe, 2’,7’-dichlorofluorescin diacetate (DCFH-DA), which could be converted to highly fluorescent dichlorofluorescein (DCF) with the presence of intracellular ROS on human fibrosarcoma cell
(HT1080). Those various antioxidant activities were compared tostandard eantioxidants such as butylated hydroxytoluene (BHT) and α-tocopherol. Among SHM exhibited the highest antioxidant activity in linoleic acid system, effective reducing power, DPPH radical scavenging, hydroxyl radical scavenging, superoxide radical scavenging, alkyl radical scavenging, inhibitory intracellular ROS. Furthermore, MTT assay showed no cytotoxicity on human embryonic
lung fibroblasts cell line (MRC-5) and human fibrosarcoma cell (HT1080). This antioxidant property depends on concentration and increasing with increased amount of sample. The results obtainedin the present study indicated that the sea horse (Hippocampus coronatus) is a potential source of natural antioxidant.