원문정보
초록
영어
In order to develop new anti-photoaging agents, we examined the antioxidant activity and the inhibitory effect of the extract of a marine product on matrix metalloproteinase-2 (MMP-2), Corallinaceae (Corallina pilulifera). It was extracted with methanol (CPM) and its inhibitory effect on MMP-2 was investigated using various antioxidant assays. The total antioxidant, DPPH
radical scavenging, reducing power and protective effect on DNA damage were evaluated. The ROS level was detected using a fluorescence probe, 2’,7’-dichlorofluorescin diacetate (DCFH-DA), which could be converted to highly fluorescent dichlorofluorescein (DCF) with the presence of intracellular ROS on human fibrosarcoma cells (HT1080). Inhibitory effect of MMP-2 was examined by gelatin zymography. The CPM extracts showed strong antioxidant activity.
100 ug/ml of CPM extract inhibited 85.6% of peroxidation level in linoleic acid system and 87.2% of DPPH radical scavenging activity, respectively, and exhibited higher antioxidant effect than the same dose of α-tocopherol. In addition, CPM showed protective effect on DNA damage cause by hydroxyl radicals and inhibited intracellular ROS levels and MMP-2 activity in human
fibrosarcoma cell line (HT1080). These results suggest that CPM may play an important role in the anti-photoaging.