원문정보
초록
영어
To investigate visceral excitatory neuropeptides of Chondrichthyes, peptides were purified from the brain of cloudy dogfish (Scyliorhinus torazame) using fish hindgut as the physiological assay system. Neuropeptides were purified from acetic acid brain extract of dogfish by using reversed phase chromatography with sep-pak C18 cartridges and high performance liquid chromatography (HPLC). One visceral excitatory neuropeptide was determined by MALDI-TOF mass spectrometry and its sequence was determined by ESI-Q-TOF mass spectrometry. Threshold concentration of Df for the excitatory effect on the visceral segment of dogfish was 0.01 / ㎍ ㎖-1㎍/㎖ and 20min or more was required for the complete recovery of intestine from the desensitization state brought by 1㎍/㎖.