원문정보
초록
영어
Cellulase activities assay for the enzyme produced from a filamentous fungus Trichoderma reesei strain QM9414 and Rut C-30 were performed. Reducing sugar released from cellulase hydrolysis was detected by dinitrosalicylic acid (DNS) method. The color developed from DNS was then measured by Digital Image Processing (DIP) technique in normal scale comparing the conventional spectrophotometric assay to the new DIP technique. Both spectrophotometric assay and DIP technique showed similar average cellulase activities with no statistically difference (P>0.05) among the 2 methods in normal scale. The cellulase activities of T. reesei QM9414 and Rut C-30 were measured, using mini-scale (5 times reduced) of DNS method comparing spectrophotometric assay to the DIP technique. The mini-scale of spectrophotometric assay and the DIP technique also displayed no difference of cellulase activities at confidence interval 95%. This study showed that the cellulase activity determination in the mini-scale by the DIP technique could rapidly be used for the determination with convenience and accuracy.