원문정보
초록
영어
Corynebacterium glutamicum, a gram positive bacterium, is widely used for the
biotechnological production of amino acids. The amine group on the surface of C. glutamicum
was found to be responsible for the electrostatic binding of anionic dye [1]. In this study,
Polyethyleneimine (PEI), with a large number of amine groups in a molecule [2], was crosslinked
with C. glutamicum, which introduced primary and secondary amine groups for
enhancing the anionic dye (RR 4) binding. The dye uptake onto PEI modified biomass was
greatly affected by the amount of polymer (PEI) and cross-linker, Glutaraldehyde (GA). Thus
PEI modification conditions were quantificationally analyzed and optimized through response
surface methodology (RSM), where PEI and cross-linker were minimized, dye uptake was
maximized. A two-level-two-factor (22) full factorial central composites design (CCD) was
performed [3], and second order polynomial models was generated to describe the effect of PEI
and cross-linker on the RR 4 uptake (R2=0.889). The optimum conditions for PEI modification
were found 1.885 g PEI and 0.52 mL GA (25 wt %), contacting with 10 g dried C. glutamicum.