원문정보
초록
영어
The epoxide hydrolase gene from M ugil cephalus (referred to as MCEH) was cloned by using rapid amplification of cDNA ends (RACE) techniques from cDNA library. The characteristic catalytic triad that is composed of Asp160, Glu378 and His366 and two tyrosines were highly conserved. Some amino acids near the active site were replaced by site-directed mutagenesis based on homology modeling in which Aspergillus niger epoxide hydrolase was used as
the template. The mutated genes were successfully expressed in Escherichia coli and the recombinant E. coli exhibited enhanced enantioselective hydrolysis activities. Acknowledgement: This work was supported by the Marine and Extreme Genome Research Center Program, Ministry of Land, Transportation and Maritime Affairs, Republic of Korea.