원문정보
초록
영어
Polydiacetylenes (PDAs), which possess unique properties that allow them to change color in
response to environmental changes such as variations in pH, temperature, and molecular
binding, have been widely investigated as signal transducers in biosensor applications. Most
PDA-based sensors reported to date have been evaluated largely on the basis of their ability
to detect purified samples, however, and their specificity has rarely been tested. In this
study, novel PDAs fabricated on polyvinylidene fluoride (PVDF) strips by photoreaction of
composite diacetylene self-assemblies were developed as biosensors, and nonspecific binding
to off-target biomolecules was assessed. A mixed PDA surface containing biotin and
ethanolamide bound the target, i.e., streptavidin, more specifically than did biotin alone. The
optimized PDA biosensor exhibited approximately 2,850-fold higher selectivity for streptavidin
relative to bovine serum albumin controls. What exactly was further prepared and showed
distinctive signals for the urine of diabetic patients compared to urine samples from
healthy/non-diabetic persons due to the concentration of microalbuminuria. To our knowledge,
this is the first strip-type biosensor fabricated with PDAs and the first PDA-based biosensor
that can effectively overcome the problem of nonspecific binding.