원문정보
초록
영어
Fermentation studies were carried out under the various fermentation conditions in 5-liter bioreactors for enhanced production of itaconic acid by a filamentous fungus, Aspergillus terreus. Itaconic acid, a dicarboxylic acid used in the manufacture of various synthetic resins is biosynthesized in a branch of the TCA cycle via decarboxylation of cis-aconitate. It was found that production level of itaconic acid considerably depend on the medium compositions of both growth and production cultures and culture temperature. The ratio of the constituent carbon, nitrogen and phosphate sources of the production medium was observed to be one of the most
important factors for the enhanced production of itaconic acid1). Therefore, for the maximal utilization of the producers' fermentation physiology, the composition of the production medium was further optimized using powerful statistical methods such as full factorial design(FFD), steepest ascent method(SAM) followed by response surface method(RSM)2). In addition, the effects of various surfactants on itaconic acid production were investigated using the optimized production medium, because it was assumed that the high productivity of itaconic acid by the
high-yielding mutants should be related to the special roles of membrane permeability of the mutant producers. In this paper, we also introduce miniaturized fermentation systems proved to be very efficient for large and mass screening of high yielding mutants.