원문정보
초록
영어
A developmental exposure to environmental neurotoxicants produces alterations in the
dopaminergic system and has been linked to neurodegenerative disorders, including
Parkinson’s disease. The present study was undertaken to elucidate polychlorinated biphenyls
(PCBs)-responsive gene expression profiles in human neuronal cells. SH-SY5Y and U937
human neuroblastoma cells were exposed to 100 nM PCBs for 0, 12, 24 and 48h in culture,
after which gene expression profiles were analyzed by the proteomics analysis and bio-cell
chip. In proteomic analysis of human neural cells, 50 different proteins showed significant
quantitative changes during the experiment, which were associated with PCBs time -
dependent manner. The results involved the characteristics of proteins, which can be
categorized into skeleton, signal transduction, cytokine, apoptosis, and reactive oxygen
species metabolism. In conclusion, results suggest that the identified proteins provide crucial
information to understanding possible mechanisms of toxicity of PCBs-induced endocrine
disruption.