원문정보
초록
영어
Nanofibers can be produced easily by using electrospinning method, which is simple, versatile and easy to handle. They also have an advantage over mesoporous media by relieving the mass transfer limitation of substrates/products due to their reduced thickness. In this presentation we prepare the nanofiber by using electrospinning for enzyme stabilization. As composition for nanofiber, we used polystyrene (PS) and poly (styrene-co-malenic anhydride) (PSMA). Enzyme-nanofibers composite was prepared by fabricating enzyme-aggregate coating on the surfaces of nanofibers. Esterase from Rhizopus oryzae was used for fabricating enzyme-nanofiber composite in this study. The enzyme aggregations coated on nanofibers were greatly stable during long-term storage. In addition, the enzymes coated on nanofibers did not lose its activity during recycling operation. These biocatalytic nanofibers were employed to biodegradation of environmental pollutants, for example Di-(2-ethylhexyl) phthalate (DEHP). Continuous biodegradation process was also possible by using the biocatalytic nanofibers in
continuous reactor due to highly stable property of enzyme coated on nanofibers. The immobilized enzyme on nanofibers, yielding high stability, creates an economical ly viable enzyme system for the use of expensive enzymes with potential applications in bioconversion, bioremediation and biosensors.