원문정보
초록
영어
Bioconversion of racemic styrene oxide to (R)-phenyl-1,2-ethanediol was studied by using two enantiocomplementary epoxide hydrolases (EHs) from Caulobacter crescentus and Mugil cephalus. C. crescentus EH (CcEH) primarily hydrolyzed (S)-styrene oxide to (R)-phenyl-1,2-ethanediol as the main product. Unlike CcEH, M. cephalus EH preferentially attacked at the terminal carbon of (R)-styrene oxide, thus leading to the formation of (R)-phenyl-1,2-ethanediol.
To improve enantiopurity and yield of the diol, the effects of the addition of detergent, temperature, product inhibition and substrate concentrations were investigated. We obtained (R)-phenyl-1,2-ethanediol with a high enantiopurity at the optimal reaction condition. Acknowledgement: This work was supported by the Marine and Extreme Genome Research Center Program, Ministry of Marine Affairs and Fisheries, Republic of Korea.